Abstract Adoptive transfer of T cells genetically engineered with a T cell receptor (TCR) is a promising cancer treatment modality that requires the identification of TCRs with good characteristics.Most T cell cloning methods involve a Manicure Kit stringent singularization process, which necessitates either tedious hands-on operations or high cost.We present an efficient and nonstringent cloning approach based on existing techniques.We hypothesize that after elimination of most nonspecific T cells, a clonotype with high quality could outcompete other clonotypes and finally form a predominant population.
This Bassinets TCR identification method can be used to clone virus-specific TCRs efficiently from cancer patients and is easily adoptable by any laboratory.